ABSTRACT
Objective To evaluate the efficacy and safety of laparoscopic pyloromyotomy (LP) or open pyloromyotomy (OP) for the treatment of congenital hypertrophic pyloric stenosis(CHPS),and to provide a basis for reasonable treatment programme for clinic.Methods Randomized control trials (RCTs) about LP and OP for the treatment of CHPS were searched using Cochrane library,PubMed,Embase,Cumulative Index To Nursing And Allied Health Literature (CINAHL),Web of Science,Chinese biomedical literature database and China National Knowledge Internet database.Study selection and Meta analysis were conducted according to the Cochrane Handbook for systematic reviews.Extracted data from the selected studies were reviewed by the reviewers and analyzed using RevMan 5.1 software.Results Five RCTs with a total of 552 patients fulfilled the inclusion criteria and were analyzed in this review.Meta analysis showed that there was no statistically significant differences in incidence of postoperative complications between LP and OP (OR =2.59,95 ％ CI:0.90-7.47).Compared with OP,the postoperative recovery eating time and postoperative hospital stay in patient with LP were significantly reduced (MD =-3.63,95％ CI:-5.19--2.06) ;MD =-12.62,95 ％ CI:-16.13--9.11).Conclusions LP result in earlier feeding,shorter hospital stay,less trauma with superior cosmetic outcome and earlier recovery of the patient than the OP.But the postoperative complication between the two surgeries still remains the topic of concern.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether Echinococcus granulosus cyst fluid-infected host liver cells had differential expression of mitogen-activated protein kinases (MAPKs) or differential cell cycle activity.</p><p><b>METHODS</b>Human liver cells cultured with different concentrations of hydatid cyst fluid (HCF) were tested by the MTT method to determine effects on proliferation. The cell cycle was assessed by flow cytometry. Western blotting was used to detect changes in protein expressions of p-ERK, PCNA, cyclin-A, cyclin-B1, cyclin-D1, and cyclin-E.</p><p><b>RESULTS</b>Forty-eight, 72 and 96 h of HCF at 15%, 30% and 60% concentrations in the cell media significantly promoted cell proliferation (F=67.845, P less than 0.01) and compared to controls (P less than 0.05). Cells exposed to 15% HCF for 48 h showed significantly induced expression of p-ERK (F=1.916, P less than 0.01), higher than controls (P less than 0.01). Cells exposed to 15% HCF for 24 h showed significantly induced expression of cyclin-Dl (F=3.901, P less than 0.01), higher than controls (P less than 0.01). Cells exposed to 15% HCF for 48 h or 30% HCF for 72 h showed significantly induced expression of PCNA (F=91.140, P less than 0.01), higher than controls (P less than 0.01). Cells exposed to 15% HCF for 48 h or 30% HCF for 72 h shed significantly induced expression of cyclin-A (F=18.587, P=0.002), higher than controls (P less than 0.01). Cells exposed to 15% HCF for either 48 h or 72 h showed significantly induced expression of cyclin-B1 (F=2.064, P less than 0.01), higher than controls (P less than 0.01). Cells exposed to 30% HCF for 96 h showed significantly induced expression of cyclin-E (F=1.068, P less than 0.01), higher than controls (P less than 0.01).</p><p><b>CONCLUSION</b>Hydatid cyst fluid exerts no inhibitory effect on primary cultured host liver cells, but may promote cellular proliferation.</p>